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Marisco (Improving Arable Production Systems By Expressing Marine Algal Rubisco In Crop Plants) is a EU-financed (Contract No. QLK3-CT-2002-01945) research project.
The project is organized from: The Swedish University of Agricultural Sciences in Uppsala (SLU) by Professor. Inger Andersson.
The overall objectives of the project are:
-The genetic, kinetic, phylogenetic and structural mapping of carbon assimilating
enzymes from marine phytoplankton/algae/ from north Atlantic/Arctic waters.
-The expression of marine algal Rubisco genes in the unicellular green alga
Chlamydomonas sp..
-Mutagenesis of Rubisco using this system (based on phenotypic selection) with
the aim to improve catalysis.
-The investigation of the possibilities of transformation of chloroplasts of
a major crop plant, such as wheat or potato, with the gene(s) of Rubisco from
marine algae with high Carbon Dioxide selectivity.
-To install a network of European laboratories specialised in Rubisco research.
Specific scientific objectives are:
-To set up routines for the collection, purification and biochemical characterisation
of Rubisco from marine algae
-To determine the sequences of these enzymes
-To study the phylogeny of Rubisco from marine algae on the basis of results
from investigations outlined above.
-To crystallise Rubisco from marine algae and collect X-ray data for the determination
of their respective 3D structures.
-To develope methods for the crystallisation of Rubisco activase for structure
determination and elucidation of the basis for activation of Rubisco.
-To clone the gene(s) of Rubisco from marine algae with a high selectivity for
Carbon Dioxide.
-To introduce Rubisco genes from marine algae into the chloroplast of Chlamydomonas.
-To develop a protocol for the introduction and expression of Rubisco genes
from marine algae in higher plants.
-To investigate the possibilities of using the engineered enzymes in cell factories.
This page is maintained by H.C. Eilertsen
Last updated 03.12.05 PROJECT TERMINATED
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